#ULOG INDUSTRIAL TRAINING ~WE DID EXTRACTION OF DNA TODAY.

For extraction of E.coli DNA we need the following.

• A buffer
• Taq polymerase
• Forward primer
• Reverse primer
• Nuclease free water
• Dntp
• Mgcl2
• The DNA we want to replicate (template)

We vortex the solution then place them in little tube like vials called PCR TUBES, then place them in the PCR machine .

The machine amplifies and replicates the DNA into thousands of copies within a few hours.

NB: The PCR machine is seen in the first picture.