I see papers describing genome editing as a simple inject-nuclease-wait for embryos to grow thing. However, how does the nucleus enter, for example blastomere nuclei,
getting through the nucleolemma
reaching condensed solenoids that possess epigenetic modification and structural factors that prevent the binding of factors?
Well, the genome editing I personally have been doing for the past few weeks involves injecting the solution in a zygote before it reaches two-cell stage, so I don't have any insights on how it works in later stages :/
Oh, that's okay. thanks for the response though :)