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I see. Not so difficult to find a different signal, but difficult to accurately identify exactly what is making that signal. I understand even if I didn’t manage to restate it very elegantly!

@flyyingkiwi you can see my latest post, its about the 16S rRNA gene sequencing and identifying a novel bacteria species.

Easy to find novel 16S rRNA. Difficult to isolate the bug responsible and create a type strain to make it an official species.

Thank you @flyyingkiwi and @effofex
16S rRNA gene sequencing is best to identify one but to find one from the source is like mining. You have to find the one among hundereds or even thousand but if you know how to find them and what characterstics a novel one could possess, it would be much easier to find the novel species.

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