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RE: What are good labs for a Developmental Biology Course?

in #stemq5 years ago

Compare the toxicity and teratogenic effects of intercalating dyes (commonly used in biomedical laboratories) using a model organism with fast generation times. Is SYBR Green safer than Ethidium Bromide (EB)? What are the effects of disposing EB in bleach as some labs do?

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Thanks for the quick reply!

That's a very good idea. We do plan to work with an immortal human cell line. The generation time isn't that fast, but it's quick enough that students can treat cells with both and assess growth after a week. We could also try this with C. elegans, but I'm not sure if we'll get a chance to work with them.

The dyes might also make DNA visible in fluorescent microscopy.

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